This side event explores ecological connectivity as a key strategy for implementing the CMS Samarkand Strategic Plan for Migratory Species and aligning it with the Kunming–Montreal Global Biodiversity Framework. It will emphasise South–South cooperation, showcasing case studies from the Amazon, Pantanal, and South Atlantic to demonstrate how regional governance can deliver global impact. The discussion will feature perspectives from Brazil, Bolivia, Chile, and Peru, highlighting cross-border collaboration and lessons from terrestrial, marine, and freshwater systems. Participants will identify political and technical elements needed to scale connectivity initiatives and foster synergies between CMS and CBD processes. Expected outcomes include high-level political messages, regional cooperation opportunities, and Brazil’s leadership in advancing a South–South connectivity agenda ahead of CBD COP17.